An Assay for Pantothenic Acid in Biologic Fluids
نویسندگان
چکیده
منابع مشابه
An assay for pantothenic acid in biologic fluids.
ANTOTHENIC ACID DEFICIENCY reduces biologic actylations and leads to a variety of pathologic changes in blood cholesterol partition and antibody production. Deficiency affects particularly the adrenal cortex, nervous system, the skin, and hair; it also affects longevity (1, 2, 3, 4). The ubiquity of pantothenic acid (as suggested by its name), makes it difficult to demonstrate deficiencies, eve...
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COMPARED WITH CHEMICAL METHODS, microbiologic assay methods for measuring vitamin activity often are more sensitive, more specific, and require less investment in equipment, and less material for assay-important considerations when biologic substances are to be analyzed (1). Thiamine is one of the few vitamins assayable chemically (2), but this technic is often impractical in clinical situation...
متن کاملThe pantothenic acid content of coenzyme A by chick assay.
Lipmann, Kaplan, Novelli, Tuttle, and Guirard (1) reported that, whereas concentrates of the coenzyme required for acetylation (coenzyme A) showed no pantothenic acid activity by the ordinary microbiological assay, considerable amounts of p-alanine were found after acid hydrolysis. From this early observation, it was suspected that the coenzyme might contain combined pantothenic acid which was ...
متن کاملAssay and distribution of bound forms of pantothenic acid.
The distribution of CoA (I), pantetheine (2, 3), and pantothenic acid (4) among a variety of natural materials is well established. However, until now, the relative amounts of these compounds present in any one substance has not been determined. Also, data on the distribution of P-pantothenic1 acid and P-pantetheine, both of which are intermediates in the biosynthesis of CoA (5), is rather meag...
متن کاملMeasurement of uric acid in biologic fluids using an ion-exchange separation.
HE MAJOR METHODS CURRENTLY IN USE for the determination of uric cid in biologic fluids are the colorimetric (1-3), the titrimetric (4), and he enzymatic (5-7). All of these methods are performed directly on the luid to be measured and are therefore subject to the effects of interfering ubstances present in these fluids. Since the colorimetric and titrimetric echnics depend on the reducing abili...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1960
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/6.1.36